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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Jul 31;1822(11):1727–1734. doi: 10.1016/j.bbadis.2012.07.014

Figure 5. RAPA RECQL4 lacks ATPase activity.

Figure 5

A, a representative phosphorimage of a TLC plate showing the relative ATPase activity of WT and RAPA RECQL4. For this assay a 61-mer ssDNA (8 pmol) was incubated with either WT or RAPA RECQL4 and 1 μCi [γ-32P] ATP (PerkinElmer Life Sciences) in helicase assay buffer with 50 μM cold ATP for 1 h at 37 °C. The protein concentrations tested were 10, 50 and 100 nM. B, Graphic representation of the quantification of three independent ATPase experiments is shown. The mean and standard deviation are reported.