Figure 4. Bim and Puma are required for deletion of OT-I thymocytes in RIP-mOVA⁺ mice.

A. Representative dot plots of CD4 and CD8 expression gated on H2-K^b-OVA tetramer⁺ thymocytes from WT or RIP-mOVA⁺ chimeras that had been reconstituted with BM from OT-I transgenic mice on WT, Bim-deficient or Puma and Bim double-deficient backgrounds. The percentages of DP, CD4SP and CD8SP gated on H2-K^b-OVA⁺ thymocytes are shown. Mean percentages (B.) and cell numbers (C.) of H2-K^b-OVA⁺ CD8SP thymocytes (±SEM) from the various chimeras. D. H2-K^b-OVA versus CD8 staining on splenocytes from the various chimeras, with the proportion of H2-K^b-OVA⁺ CD8⁺ T-cells shown. Mean percentages (E.) and numbers (F.) of H2-K^b-OVA⁺ CD8⁺ splenocytes (±SEM) from the various chimeras. PB denotes Puma and Bim double-deficient. Groups composed of 3–4 mice were compared using student’s t-test; * p<0.05, ** p<0.01, *** p<0.001. Data from one of two experiments are shown.