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. Author manuscript; available in PMC: 2013 Sep 21.
Published in final edited form as: Immunity. 2012 Sep 6;37(3):451–462. doi: 10.1016/j.immuni.2012.05.030

Figure 5. Both Bim and Puma are required for the deletion of mature CD4SP OT-II thymocytes in RIP-mOVA+ mice.

Figure 5

A. Representative dot plots of CD4 and CD8 expression gated on Vα2/Vβ5+ thymocytes from RIP-mOVA− or RIP-mOVA+ chimeras that had been reconstituted with BM from OT-II transgenic mice on WT, Bim-deficient or Puma and Bim double-deficient backgrounds. The percentages of Vα2/Vβ5+ CD4SP are shown. Mean percentages and cell numbers of Vα2/Vβ5+ CD4SP thymocytes (B.) or Vα2/Vβ5+ CD4+ splenocytes (C.) (±SEM) from the various chimeras. D. Qa2 versus CD24 expression on Vα2/Vβ5+ CD4SP from the various chimeras. Groups composed of 3–4 mice were compared using student’s t-test; * p<0.05, ** p<0.01. Data from one of two experiments are shown.