Figure 3.

Distinct Kinetic Properties of Remodeled A1/A2 Receptors

(A) Brief-pulse desensitization of AMPAR responses during a 100 Hz application (1 ms pulses) of L-Glu (10 mM). Traces are normalized to the peak current of the first pulse. Activity deprivation was associated with less depression of current amplitudes during the burst. (B) Summary of the group data for the assay in (A). Quantification of the burst responses by normalizing each response to the initial response (I_x / I₁) is shown. Since responses summated, the current amplitude for each peak response was measured from the local preceding baseline (see Figure S4A). Number of patches for each treatment was 15 (CA1: CTRL), 15 (CA1: TTX), and 4 (CA3: CTRL). Values represent the mean ± SEM. (C) The stimulation protocol from (A) (train of 1 ms pulses of 10 mM L-Glu at 100 Hz) applied to recombinant A1/A2 heteromers. Note the faster AMPAR deactivation compared to CA1 patches caused by the absence of auxiliary subunits. (D) Group data for the assay in (C) as described in (B). Number of patches presented here is 6 for A1o/A2i and 6 for A1i/A2o.