Table 3.
Gene expression changes following a hepatoprotective dose of zinc in mice
| Symbol | GeneBank# | Encoded proteins | Ratio Zn/control array | Ratio Zn/Control RT-PCR |
|---|---|---|---|---|
| Acute phase protein genes | ||||
| Mt1 | BC027262 | Metallothionein-1 | N/A | 514 |
| Mt2 | NM_008630 | Meathiothinein-2 | N/A | 1020 |
| Hmox1 | M33203 | Haeme oxygenase-1 | 1.8 | 2.2 |
| Stat3 | U06922 | Signal transduction/activator 3 | 2.3 | 2.9 |
| Egr1 | M20157 | Early growth response 1 | 2.6 | 2.3 |
| Cxcl1 | NM_008176 | Chemokine (C-X-C motif) ligand 1 | 1.3 | 3.2 |
| Cp | U49430 | Ceruloplasmin | 1.3 | 2.9 |
| Hspa1b | M35021 | Heat-shock protein 70 | 1.2 | 1.9 |
| c-jun | J04115 | Jun oncogene | 1.3 | 1.9 |
| Antioxidants | ||||
| Nrf2 | BC026943 | Nuclear reactor factor Nrf2 | 2.1 | 1.4 |
| Nqo1 | BC004579 | NAD(P)H dehydrogenase, quinone 1 | 1.5 | 2.1 |
| Gclm | NM_002061 | Glutamate-cysteine ligase, modifier unit | N/A | 3.0 |
| Gstp1 | D30687 | Glutathione S-transferase-π | 0.7 | 0.8 |
| Gpx3 | U13705 | Glutathione peroxidase 3 | 0.9 | 1.8 |
| Sod1 | NM011434 | Superoxide dismutase 1 | 1.0 | 1.7 |
| Cytokines and inflammatory mediators | ||||
| Il1b | NM_008361 | Interleukin-1β | 0.8 | 1.4 |
| Il6 | J03783 | Interleukin-6 | 0.7 | 1.0 |
| Il10 | M37897 | Interleukin 10 | 1.2 | 1.1 |
| Tnf | NM_013693 | Tumour necrosis factor (TNF)-α | 0.9 | 1.2 |
| Tnfrsf1b | M59378 | TNF receptor 1 | 1.3 | 1.1 |
| Nos2 | M87039 | Inducible nitric oxide synthetase | 1.4 | 1.2 |
| Ptgs2 | M64291 | Prostaglandin synthase, Cox2 | 1.2 | 1.1 |
| Metabolic enzymes | ||||
| Cyp2j5 | NM_010007 | Cytochrome P450 2J5 | 0.5 | 0.8 |
| Cyp3a11 | X60452 | Cytochrome P450 3A11 | 0.4 | 0.5 |
| Cyp4a10 | AB018421 | Cytochrome P450 4A10 | 0.6 | 0.5 |
| Cyp4a14 | Y11638 | Cytochrome P4504A11 | 0.6 | 0.8 |
| Cyp2e1 | L11650 | Cytochrome P450 2E1 | 0.8 | 1.4 |
| Cyp2f2 | M77497 | Cytochrome P450 2F2 | 0.8 | 1.2 |
| Ugt1a2 | D87866 | UDP-glucuronosyltransferase 1A2 | 0.7 | 0.8 |
| Bhmt | AF033381 | Betaine homocysteine Me-transferase | 0.5 | 0.8 |
| Growth and cell proliferation | ||||
| Ccnd1 | M64403 | Cyclin D1 | 2.4 | 2.3 |
| Pcna | X53068 | Proliferate cell nuclear antigen | 2.3 | 1.5 |
| Igfbp1 | X81579 | Insulin-like factor binding protein 1 | 2.2 | 2.5 |
Mice were treated with Zn (100 μmol/kg, s.c. daily for 4 days). Data are mean ±SEM (n = 3 for microarrays and n = 5 for real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of individual mouse). Bold indicates significantly different from controls P <0.05.
N/A: not available on the array.