Table II.
Effects of NADH, p-CMPS, and NADH plus p-CMPS on the NADH-FeCNR activity of the enzymes ES, ET1, and ET2
| Enzyme | NADH-FeCNR Activity
|
||
|---|---|---|---|
| NADH | p-CMPS | NADH + p-CMPS | |
| % | |||
| ES | 92.0 ± 5.0 | 88.5 ± 4.5 | 77.5 ± 13.0 |
| ET1 | 99.5 ± 5.5 | 92.0 ± 6.5 | 87.0 ± 4.5 |
| ET2 | 114.5 ± 4.5 | 89.5 ± 5.0 | 92.0 ± 4.0 |
The enzymes were incubated at 30°C for 20 min with 0.5 mM NADH, 0.05 mM p-CMPS, or 0.5 mM NADH plus 0.05 mM p-CMPS prior to the activity assays. The values indicate activity percentages in relation to nontreated samples (see Table I) and are means ± se of three to five independent assays.