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. 2012 Nov 19;2:874. doi: 10.1038/srep00874

Figure 2. Transient expression of mGFP5 and GusPlus in N. benthamiana leaves by using TMV-Gate vectors.

Figure 2

(A) N. benthamiana leaf infiltrated with A. tumefaciens carrying empty vector or viral expression vector encoding mGFP5 with or without translational fusion to either HA, 3xHA, FLAG, 3xFLAG, c-myc, 3xc-myc or 2xStrep-tag II-6xHis-Xpress epitope tag(s) was illuminated under UV light at 5 DPI and photographed. Inset shows immunoblot detection of 2xStrep-tag II-6xHis-Xpress epitope-tagged mGFP5 (in silico predicted size of 37.8 kDa) in crude extract using anti-6xHis antibody. (B) N. benthamiana leaf discs from the area infiltrated with A. tumefaciens carrying empty vector (pTK251) or viral expression vector encoding 2xStrep-tag II-6xHis-GusPlus (pSK154) were excised 5 DPI and stained for GUS activity. Inset in right panel shows immunoblot detection of 2xStrep-tag II-6xHis-Xpress epitope-tagged GusPlus (in silico predicted size of 81.5 kDa) in crude leaf extract using anti-6xHis antibody. (C) N. benthamiana leaf infiltrated with various dilutions of a cell suspension of A. tumefaciens at OD600 of 1.0 carrying pSK133 (pMW388 encoding mGFP5) and photographed with UV illumination at 5 DPI.