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Effect of mutations in ZNRF2 on its binding to 14-3-3. (A) Wild-type ZNRF2-GFP or single/double serine to alanine mutants were isolated from transfected HEK293 cells that had been stimulated with IGF1, PMA or forskolin as indicated. The ZNRF2-GFP proteins were tested for their phosphorylation and 14-3-3 binding, as for Fig. 1. (B) Similar to A, but including mutated GST-ZNRF2 phosphorylated with PKBα, SGK1, p90RSK, PKA, PKCα and PKCζ, as indicated. WT, wild type.
