Fig. 5.

The expression of CPC and chromosome alignment in MLL5-siRNA-treated cells can be rescued by overexpression of FLAG-MLL5. (A) The lack of expression of CPC at G2 caused by MLL5 knockdown was rescued by overexpression of FLAG-MLL5 or HA-Borealin but not FLAG-MLL5ΔCD. The efficiency of the rescue was assessed and the percentages of cells expressing CPC are presented in the bar chart (n = 60 cells per sample). (B) The CPC localization and chromosome alignment defects observed in MLL5-siRNA-treated cells were rescued by FLAG-MLL5 and HA-Borealin overexpression. The rescue experiments were repeated three times, and the average percentages of cells (normal chromosome alignment, bipolar misalignment and multipolar misalignment) were determined (n = 100 cells per sample). Scale bars: 10 µm.