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HPLC purification of UMP/CMP kinase. The GST-UMP/CMP kinase fusion protein was digested with enterokinase while still attached to the glutathione-Sepharose beads. The eluate was applied to a 250- × 10-mm, i.d., reverse-phase C18 column. A214 was monitored. The eluted protein peak containing UMP/CMP kinase activity was collected (B = 41%). The hatched area under the peak illustrates the collected pool of UMP/CMP kinase.
