Table 2.
Immunohistochemical panel
| Antigen/antibody/clone | Type | Company | Dilution | Antigen recovery |
|---|---|---|---|---|
| Cytokeratin (AE1/AE3:M3515 and CAM5.2) | mm | Dako, Carpinteria, CA Boehringer Mannheim Biochemicals, Indianapolis, IN |
1:40 1:8 |
CC1, 30 min |
| CK5/6 (D5/16 B4) | mm | Dako | 1:25 | E2, 20 min |
| CK7 (OV-TL-12/30) | mm | Dako | 1:200 | CC1, 30 min |
| CK20 (KS20.8) | mm | Ventana Medical Systems, Tucson, AZ | Neat | CC1, 30 min |
| Epithelial membrane antigen (E29) | mm | Ventana | Neat | CC1, 30 min |
| CAM5.2 (CK8/18) | mm | Covance, Princeton, NJ | 1:8 | CC1, 30 min |
| p63 (7jul) | mm | Leica Microsystems, Buffalo Grove, IL | 1:40 | E2, 30 min |
| CEA (CLO1-cea ab-1) | mm | Lab Vision/NeoMarkers, Fremont, CA | 1:250 | CC1, 30 min |
| Calponin | mm | Abcam, Cambridge, England | Neat | CC1, 30 min |
| Muscle specific actin (HHF35) | mm | Enzo Life Sciences, Farmingdale, NY | 1:100 | CC1, 30 min |
| Smooth muscle actin (asm-1) | mm | Leica, Wetzlar, Germany | 1:200 | E2, 20 min |
| Smooth muscle myosin heavy chain (SMMS1) | mm | Dako | 1:100 | CC1, 30 min |
| S100 protein | rp | Dako | 1:2,000 | CC1, 30 min |
| CD117 (C-Kit) | rp | Dako | 1:400 | CC1, 30 min |
| GFAP (6F2) | mm | Dako | 1:200 | CC1, 30 min |
| p53 (DO-7) | mm | Dako | Neat | CC1, 30 min |
| Ki-67 (MIB-1) | mm | Dako | 1:100 | CC1, 30 min |
mm mouse monoclonal, rp rabbit polyclonal