Fuse-core column stability
Column: 2.1 × 100 mm; Particles: 2.7 μm 400 Å - C8; Mobile phase - A: water/aqueous 0.1% trifluoroacetic acid; B: 70% acetonitrile/ 30% aqueous 0.1% trifluoroacetic acid; Gradient: 9 to 55% B in 10 min; Flow rate: 0.5 mL/min; Temperature: 60 °C; Injection: 1 μL; Instrument: Shimadzu Prominence; Detection: 220 nm
Peak widths measured at half-height for selected compounds (min). Peak identities as stated in Figure 6.