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Effect of pore size on separation of myosin
Columns: 2.1 × 100 mm as shown; Temperature: 80°C; Mobile phase; A = water/0.1% trifluoroacetic acid; B = acetonitrile/0.1% trifluoroacetic acid; Gradient: 35 – 65% B in 15 min.; Flow rate: 0.45 mL/min; Detection: 215 nm; Injection: 1 μL; Instrument: Agilent 1200 SL.
