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. Author manuscript; available in PMC: 2013 Nov 16.
Published in final edited form as: J Chromatogr A. 2012 Sep 25;1264:22–30. doi: 10.1016/j.chroma.2012.09.052

Figure 3.

Figure 3

Effect of bonded phase on proteins

Columns: 4.6 × 100 mm; Particles: 2.7 μm, 400 Å; Temperature: 60 °C; Mobile phase – ES-C18, apomyoglobin: 38.6% acetonitrile/61.4% water/0.1% trifluoroacetic acid ES-C8, apomyoglobin: 37.7% acetonitrile/62.3% water/0.1% trifluoroacetic acid C4, apomyoglobin: 39.4% acetonitrile/60.6% water/0.1% trifluoroacetic acid ES-C18, carbonic anhydrase: 41% acetonitrile/59% water/0.1% trifluoroacetic acid ES-C8, carbonic anhydrase: 39.76% acetonitrile/60.24% water/0.1% trifluoroacetic acid