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Effect of protein size
Columns: 4.6 × 100 mm; Particles: 2.7 μm, 400 Å, ES-C8; Temperature: 60 °C; Mobile phase – ribonuclease A: 23.9 % acetonitrile/76.1% aqueous 0.1% trifluoroacetic acid; k = 3.6 apomyoglobin: 28.3 % acetonitrile/61.7 % aqueous 0.1% trifluoroacetic acid; k = 3.2 enolase: 42% acetonitrile/ 58 % aqueous 0.1 % trifluoroacetic acid; k = 3.9
