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. Author manuscript; available in PMC: 2013 Nov 1.

Published in final edited form as: Sens Actuators B Chem. 2012 Aug 19;174:245–252. doi: 10.1016/j.snb.2012.06.095

Real-time detection of Mtb (8.7×10⁶ cells/mL) with rabbit polyclonal antibody α-LAM (A) and with guinea pig polyclonal antibody anti-H37Rv (B). PBS flow is indicated by the shaded region. Steps 1–3 depict the building of the sensing layers and step 4 depicts the detection of Mtb. The total mass detected in each step is as follows: (A-1) 253 ng protein A (A-2) 10 ng BSA (A-3) 51 ng α-LAM (A-4) 12 ng Mtb (B-1) 207 ng protein A (B-2) 26 ng BSA (B-3) 130 ng anti-H37Rv (B-4) 9 ng Mtb.

Real-time detection of Mtb (8.7×10⁶ cells/mL) with rabbit polyclonal antibody α-LAM (A) and with guinea pig polyclonal antibody anti-H37Rv (B). PBS flow is indicated by the shaded region. Steps 1–3 depict the building of the sensing layers and step 4 depicts the detection of Mtb. The total mass detected in each step is as follows: (A-1) 253 ng protein A (A-2) 10 ng BSA (A-3) 51 ng α-LAM (A-4) 12 ng Mtb (B-1) 207 ng protein A (B-2) 26 ng BSA (B-3) 130 ng anti-H37Rv (B-4) 9 ng Mtb.