Table 2.
Concentration of total events and gated events, and intact cell concentration and percentage(expressed as a proportion of gated event concentration) in zebrafish sperm samples in HBSS or C-F HBSS containing 0% (control), 10%, and 20% MeOH or DMSO (treatment),and after centrifugation and re-suspension in HBSS or C-F HBSSto remove cryoprotectants (washed).
| Total events (×105 ml−1) |
Gated events (×105 ml−1) |
Intact cells (×105 ml−1) |
Intact cells (%) |
|||||
|---|---|---|---|---|---|---|---|---|
| HBSS | Treatment | Washed | Treatment | Washed | Treatment | Washed | Treatment | Washed |
| 0% (Control) | 10.2 ± 0.3 | 9.6 ± 0.2 | 8.8 ± 0.3 | 8.6 ± 0.2 | 8.0 ± 0.3 | 8.0 ± 0.2 | 91.0 ± 0.3 | 93.1 ± 0.4* |
| 10% MeOH | 10.0 ± 0.4 | 9.1 ± 0.3 | 8.3 ± 02 | 8.2 ± 0.3 | 7.4 ± 0.2 | 7.4 ± 0.3 | 90.2 ± 0.5 | 90.4 ± 0.7 |
| 10% DMSO | 9.1 ± 0.3 | 8.6 ± 0.4 | 7.3 ± 0.3# | 7.8 ± 0.4 | 6.6 ± 0.3# | 3.9 ± 0.3*# | 90.9 ± 0.3 | 50.3 ± 1.5*# |
| 20% MeOH | 11.4 ± 0.3 | 8.4 ± 0.3* | 4.0 ± 0.3# | 7.6 ± 0.4* | 2.9 ± 0.2# | 5.4 ± 0.4*# | 72.0 ± 1.7# | 70.6 ± 2.9# |
| 20% DMSO | 16.6 ± 0.3# | 8.2 ± 0.4* | 4.6 ± 0.3# | 7.5 ± 0.4* | 2.0 ± 0.2# | 1.8 ± 0.1# | 43.3 ± 2.4# | 23.9 ± 0.6*# |
|
| ||||||||
| Calcium–Free HBSS | ||||||||
| 0% (Control) | 10.7 ± 0.3 | 9.9 ± 0.5 | 9.7 ± 0.3 | 9.4 ± 0.4 | 9.0 ± 0.3 | 8.8 ± 0.4 | 92.3 ± 0.2 | 93.7 ± 0.5 |
| 10% MeOH | 10.1 ± 0.3 | 9.7 ± 0.3 | 9.2 ± 0.3 | 8.5 ± 0.3 | 8.5 ± 0.3 | 8.4 ± 0.3 | 91.8 ± 0.2 | 92.1 ± 0.6 |
| 10% DMSO | 9.9 ± 0.2 | 8.4 ± 0.6 | 8.8 ± 0.2 | 7.9 ± 0.5 | 8.0 ± 0.2* | 5.1 ± 0.5*# | 90.7 ± 0.5* | 63.6 ± 2.1*# |
| 20% MeOH | 9.0 ± 0.2# | 9.3 ± 0.5 | 8.1 ± 0.2# | 8.8 ± 0.5 | 6.0 ± 0.2*# | 7.3 ± 0.5* | 73.9 ± 1.9*# | 82.6 ± 1.1*# |
| 20% DMSO | 8.3 ± 0.3# | 8.3 ± 0.3 | 6.9 ± 0.3# | 7.8 ± 0.3 | 5.8 ± 0.3*# | 2.6 ± 0.3*# | 83.5 ± 1.0*# | 33.5 ± 0.7*# |
*
Indicates significant difference between washed and corresponding treatment values (P < 0.05).
#
Indicates significant difference between control and treatments within in the same column and medium (P < 0.05).