Table 4.

Percentage of extracted proteins.

Flow rate	α = 180°	i	P value
0.25 mL/second	32.4%	32.4%	Nonsignificant
0.50 mL/second	37.4%	36.7%	Nonsignificant
1 mL/second	42%	42.9%	Nonsignificant

Note: Bevel opening toward the port bottom (α = 180°) with a centered versus off-centered needle insertion point.