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. 2012 Nov 19;3:254. doi: 10.3389/fpls.2012.00254

Figure 2.

Figure 2

CEL-HYB1 and CEL-HYB1-CBM visualized by SDS-PAGE and Western blotting. Protein production from Pichia pastoris_CEL-HYB1 (A) and P. pastoris_CEL-HYB-CBM (C) induced with 0.5% methanol; heterologous protein excreted in the supernatant was visualized using SDS-PAGE and Coomassie Brilliant Blue staining. Production of CEL-HYB1 (B) and CEL-HYB1-CBM (D) was confirmed by Western blot with the c-myc antibody. Culture supernatant was harvested at the specified times (T = 0, 6, 12, 24, 48, 72, and 96 h; Neg = negative control; M = PageRuler™, Fermentas) after induction with 0.5% methanol.