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. 2012 Oct 4;287(47):39412–39418. doi: 10.1074/jbc.M112.413518

FIGURE 3.

FIGURE 3.

Capillary assays toward alanine and glutamine show bacterial accumulation over a range of attractant concentrations. The wild-type McpC chemoreceptor is shown in black (Δ10 mcp amyE::mcpC), the E115A mutant (Δ10 mcp amyE::mcpC E115A) is shown in dark gray, and the K195A mutant (Δ10 mcp amyE::mcpC K195A) is shown in light gray. Accumulation to alanine is indicative of direct ligand binding, whereas the results for glutamine are an example of indirect ligand binding. The results of capillary assays to all 20 amino acids are summarized in Table 1.