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. 2012 Sep 28;287(47):39812–39823. doi: 10.1074/jbc.M112.406520

FIGURE 4.

FIGURE 4.

p38 inhibits the degradation of c-Met. A and B, p38 inhibition and knockdown promote the degradation of c-Met. After treatment with SB203580 (SB, 10 μm) for 1 h or P38 siRNA for 60 h, QBC939, RBE, and HCCC-9810 cells were treated with CHX (10 μm) for the indicated time periods and then subjected to Western blot analysis. C and D, constitutively active MKK6 inhibits the degradation of c-Met. After MKK6 was transfected for 24 h, HepG2 cells were treated with CHX (10 μm) for another 48 h and then subjected to Western blot analysis. E, c-Met inhibition inhibits c-Met degradation induced by p38 inhibition. After treatment with CHX (10 μm) for 48 h with or without SB203580 (10 μm) and PF-2341066 (PF, 100 nm) preincubation for 1 h, QBC939, RBE, and HCCC-9810 cells were subjected to Western blot analysis.