FIGURE 5.

c-Met promotes proliferation and invasion of human cholangiocarcinoma cells. A, c-Met inhibition inhibits human cholangiocarcinoma cell proliferation. QBC939, RBE, and HCCC-9810 cells were treated with PF-2341066 (PF, 50/100 nm) for the indicated time periods. Cell viability was determined by a CCK8 assay. B and C, c-Met inhibition and knockdown suppresses migration and invasion of human cholangiocarcinoma cells. The migration (C) and invasion (D) of QBC939, RBE, and HCCC-9810 cells with or without PF-2341066 (50/100 nm) and siMet treatment were analyzed using a Transwell assay. Columns, mean of three individual experiments; bars, S.E. *, significantly different from control value. D, the effects of PF-2341066 and C-MET siRNA on c-Met inhibition and knockdown were measured by Western blot.