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. 2012 Sep 27;287(47):39554–39563. doi: 10.1074/jbc.M112.408625

FIGURE 1.

FIGURE 1.

Increased susceptibility of Adamts12-deficient mice to colitis. A, increased body weight reduction of Adamts12−/− mice during colitis. Weights are expressed as means of individual data relative to initial body weight of each animal. B, colon length at time of sacrifice prior or during 2% DSS treatment. C, levels of IL-6 in colonic lavage as determined by ELISA. D, microscopic images of colonic mucosa stained with hematoxylin and eosin at day 8 after DSS treatment in mice from both genotypes. Ulcerated surface, more abundant in KO tissue, is indicated with red arrows. E, percentage of colonic surface affected by ulcers at days 0, 3, 6, 8, and 16. F, Kaplan-Meier plot of Adamts12+/+ and Adamts12−/− during DSS-induced colitis. Comparison of survival curves for both genotypes using the Gehan-Breslow-Wilcoxon test revealed that mortality was significantly higher in KO animals (p value = 0.0454). G, representative images of MPO staining in DSS-treated colons at day 8. Red arrows indicate some of the MPO-positive cells. H, quantification of MPO-positive brown cells per field in affected mucosa at days 6 and 8 of treatment. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Results are expressed as means ± S.E. (error bars).