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. 2012 Sep 27;287(47):39554–39563. doi: 10.1074/jbc.M112.408625

FIGURE 3.

FIGURE 3.

Identification of differential proteins in colon from DSS-treated Adamts12+/+ and Adamts12−/− mice. A, representative DiGE image with tissue extracts from Adamts12+/+ (CyDye 3, green) and Adamts12−/− (CyDye 5, red) mice. Some of the differential proteins are indicated. B, differentially produced proteins identified by MALDI-ToF confirmed by two-dimensional Western blotting. In each blot, uni-dimensional experiments of both Adamts12−/− and Adamts12+/+ tissues are identifiable on the right. β-Actin was used as a loading control. C, Western blots detecting protein levels of those candidates identified in DiGE analysis in three representative animals per genotype and time point. S100A8, S100A9, calprotectin, and hemopexin were found to be accumulated in the absence of Adamts-12 at days 6 and 8 in two different sets of three Adamts12−/− mice compared with three Adamts12+/+ animals. β-Actin was used as a loading control.