TABLE 1.
Overview of the chimeric and mutant Ca2+-pump constructs studied
| Construct | Description | Sequence of C-terminal amino acid residues |
|---|---|---|
| S1a | Rabbit SERCA1a wild type | 988ARNYLEG |
| S2a | Human SERCA2a wild type | 987ARNYLEPAILE |
| S2b | Human SERCA2b wild type | 987ARNYLEPGKECVQPATKSCSFSACTDG ISWPFVLLIMPLVIWVYSTDTNFSDMFWS |
| S2b-T1032* | S2b terminated before Thr1032 | 987ARNYLEPGKECVQPATKSCSFSACTDG ISWPFVLLIMPLVIWVYS |
| S2b-MFWS-AAAA | S2b with the C-terminal last four residues (MFWS) substituted with alanines | 987ARNYLEPGKECVQPATKSCSFSACTDG ISWPFVLLIMPLVIWVYSTDTNFSDAAAA |
| S1a2b | S1a with the C terminus of S2b | 988ARNYLEPGKECVQPATKSCSFSACTDG ISWPFVLLIMPLVIWVYSTDTNFSDMFWS |
| S1a2b-T1032* | S1a with the C terminus of S2b, terminated before Thr1032a | 988ARNYLEPGKECVQPATKSCSFSACTDG ISWPFVLLIMPLVIWVYS |
| S1a2b-L7/8 | S1a with the C terminus and the L7/8 luminal loop of S2b | Same as S1a2b |
| S1a-L7/8 | S1a with the L7/8 luminal loop of S2b | Same as S1a |
a Note that “1032” here refers to S2a/S2b numbering. Due to the presence of an extra amino acid in the nucleotide binding domain of S1a relative to S2a/S2b the S1a2b-T1032* chimera is actually one residue longer than S2b-T1032*.