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. 2012 Oct 1;287(47):39564–39577. doi: 10.1074/jbc.M112.346528

FIGURE 1.

FIGURE 1.

Interaction of GOLPH3 with C2GnT1 CT in vitro. A, GOLPH3 Western blot of the pulldown from KG1a cell lysate with biotinylated control or C2GnT1 1–20-aa peptide. B, GOLPH3 Western blot analysis of the pulldown from the lysate of BL21 bacteria transformed with GST-GOLPH3 cDNA and from purified recombinant GOLPH3 using biotinylated control or C2GnT1(1–20 aa) peptide. C, C2GnT1 Western blot analysis of the pulldown from KG1a cell lysate using GST (control) or GOLPH3-GST immobilized on glutathione-resin.