FIGURE 1.

Down-regulated menin expression was correlated with up-regulated Ras. A–D, sections from paraffin-embedded adjacent normal tissues and cancer tissues were stained with antibodies against menin (A and B) and Ras (C and D) for IHC staining, respectively, Original magnification, ×200. E and F, the expression of menin, detected by real time qRT-PCR (E) and Western blot (F), was increased in A549 cells that were transfected with shRNA vector against K-Ras compared with luciferase. G, menin expression, detected by Western blot, was reduced in K-Ras overexpression A549 cells.