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. 2012 Oct 1;287(47):40003–40011. doi: 10.1074/jbc.M112.382416

FIGURE 2.

FIGURE 2.

Down-regulated menin expression was partly associated with promoter DNA methylation. A, the scheme shows the CpG islands of MEN1 promoter that is the aim of the MS-PCR primer. B, MS-PCR analysis of the MEN1 promoter methylation of lung cancer tissue and adjacent normal tissue, obtained from the surgeries of six patients. The methylation/unmethylation of normal samples was set to 1 (n = 6). C, the representative bisulfite sequencing chromatograms of cancer and normal tissues in the two MEN1 promoter CpG sites. D, DNA methylation analysis by bisulfite cloning and sequencing. The analysis was carried out for two paired cancer and normal tissues bisulfite sequencing. Each circle in each row represents a single CpG site. Each line represents an individually sequenced clone, and circles represent CpG residues. White and black circles represent unmethylated and methylated CpG sites, respectively. E, A549 cells were either treated or not treated with DNA methylation inhibitor 5-aza-dc (5 μm). Real time qRT-PCR was performed to detect the MEN1 mRNA expression. *, p < 0.05 versus control. DMSO, dimethyl sulfoxide.