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. 2012 Sep 28;287(47):39834–39841. doi: 10.1074/jbc.M112.371641

FIGURE 1.

FIGURE 1.

CatB ablation increases levels of Aβ but does not affect hAPP processing in hAPPWT mice. A and B, ELISA measurements of hippocampal Aβ1–x (A) and Aβ1–42 (B) in 3.5-month-old hAPPWT/catB+/+, hAPPWT/catB+/−, and hAPPWT/catB−/− mice. The levels of Aβ1–x were elevated significantly by CatB deletion (n = 9–13 mice/genotype). **, p < 0.01 (one-way ANOVA with Tukey's post hoc test). C and D, ELISA measurements of cortical Aβ1–x (C) and Aβ1–42 (D) in 3.5-month-old hAPPWT/catB+/+, hAPPWT/catB+/−, hAPPWT/catB−/− mice. The levels of Aβ1–x and Aβ1–42 were elevated significantly by CatB deletion (n = 7–10 mice/genotype). *, p < 0.05; **, p < 0.01 (one-way ANOVA with Tukey's post hoc test). E, ELISA measurement of cortical s-APPβ in 3.5-month-old hAPPWT/catB+/+, hAPPWT/catB+/−, and hAPPWT/catB−/− mice. CatB deletion did not affect s-APPβ levels (n = 6 mice/genotype). F, representative Western blot analyses of hAPP metabolic fragments in the cortices of 3.5-month-old hAPPWT/catB+/+ and hAPPWT/catB−/− mice. G, quantification of the levels of FL-hAPP (normalized to those of GAPDH) and α- and β-CTFs (normalized to those of FL-hAPP). CatB deletion did not affect the levels of hAPP fragments (n = 9–12 mice/genotype). Values are means ± S.E. (A–E and G).