FIGURE 3.

N-Myristoyltransferase antagonizes calcineurin activity. A, the CDRE-lacZ reporter gene was transformed into nmt1–181 or isogenic wild type yeast. Quantitative β-galactosidase assays were conducted following overnight growth at 30 °C in YPD (pH 5.5) supplemented with CaCl₂ (in mm) as indicated. B, nmt1–181 cnb1Δ yeast harboring the CDRE-lacZ reporter gene were transformed with plasmids expressing wild type Cnb1, Cnb1-G2A, or Cnb1-G2E. Yeast were grown in YPD (pH 5.5) in the presence or absence of 100 mm added extracellular CaCl₂ for 4 h prior to harvesting for quantitative b-galactosidase assays. Data shown are average of quadruplicates ± S.D.