FIGURE 5.

Myristoylation promotes calcineurin membrane association. Cnb1Δ yeast expressing wild type Cnb1 (WT), Cnb1-G2A (G2A), or Cnb1-G2E (G2E) were grown in YPD (pH 5.5) for 4 h prior to lysis and ultracentrifugation to yield supernatant versus pellet fractions. Wildtype yeast were stimulated by the addition of 100 mm CaCl₂ to media 15 min prior to harvesting as indicated (+Ca²⁺). Protein samples were separated by SDS-PAGE and probed with anti-Cnb1. T, total protein; S, supernatant; P, pellet.