FIGURE 2.
Increased expression of SP-D mRNA in the bladder of the UPEC infection model in mice. A, J96 (108 cfu) or saline was injected into the bladder of female C57BL/6 mice, and the external urethral orifices were clamped for 2 h. Twenty-four hours after infection, the bladders from infected and non-infected mice and the lungs from non-infected mice were excised and used for detection of SP-D mRNA by RT-PCR as described under “Experimental Procedures.” Representative results are shown. B, densitometric analysis of the PCR bands shown as the ratio of SP-D/GAPDH. The data shown are the means ± S.D. (error bars) from three separate experiments. *, p < 0.05 when compared with uninfected bladder.