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Complementation of phage T7Δ5 growth by gp5. Complementation assays were performed as described under “Experimental Procedures.” In each panel E. coli A307 (trxA−) carrying a pair of plasmids expressing the different species of gp5 and wild-type trx was infected by phage T7Δ5 (lacking gene 5). After incubation at 37 °C for 6 h, phage plaques were photographed. Different gp5 species are wt gp5 (A), gp5-C275S (B), gp5-C313S (C), and gp5-C275S/C313S (D).
