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Mobility gel-shift assay of binding of gp5 wt and gp5-C313S to primer-DNA template. Binding and gel analysis was performed as described under “Experimental Procedures.” The picture shows gel retardation of 32P-labeled linear primer-DNA template as a function of increasing concentrations of protein (gp5 wt or gp5-C313S: 0–10 μm) as indicated.
