FIGURE 4.
5F-Fuc reduces cell surface sLeX expression. A, HepG2 cells treated with (black) or without (gray) 50 μm 5T-Fuc were incubated in the presence of anti-sLeX antibodies (CSLEX-1) or isotype-matched controls (red) followed by fluorescein-conjugated anti-IgM or streptavidin. The number of positively stained cells was assessed by flow cytometry, and the mean fluorescence intensity (MFI) for each sample was calculated (B). Values shown (n = 3) are mean ± S.E. 5T-Fuc causes a significant decrease in the average MFI of 5T-Fuc-treated cells. *, p < 0.05; **p < 0.01; ***, p < 0.0005. 5T-Fuc also significantly reduced the expression of fucosylated glycoconjugates (AAL) and LeX (FH-2) but not core fucosylated ones (LCA) in HepG2 cells (B). Similar results were obtained with HL-60 cells (C). Histograms for all conditions are reported in the supplemental information.