FIGURE 4.
Rit regulates p38-MSK1/2-CREB signaling in MEFs. A, H2O2-induced MSK1/2 and CREB phosphorylation is blunted in Rit−/− MEFs. Primary MEFs (passage 4) were serum-starved (2 h) prior to H2O2 (100 μm) stimulation for the indicated duration. Cell lysates were subjected to immunoblotting with indicated antibodies. Results are representative of three individual experiments using MEFs established from different litters. B, Rit activates p38-MSK1/2-CREB signaling. Immortalized WT-MEFs were transfected with either Flag-EV or Flag-RitQ79L (2 μg) after pretreated with SB203580 (10 μm), PD98059 (10 μm), or Ro-31–8220 (2.5 μm) for 30 min. Vehicle DMSO pretreatment was used as control. MEFs (60 h post-transfection) were serum-starved (3 h), cell lysates prepared, and subjected to immunoblot analysis. The results are representative of two different experiments.