FIGURE 2.

Hydrolysis of bovine elastin by pseudoalterin. Ten milligrams of bovine elastin in 0.5 ml of 50 mm Tris-HCl (pH 9.0) containing 10 μg of pseudoalterin was incubated at 25 °C with continuous stirring. Bovine elastin in 50 mm Tris-HCl (pH 9.0) without pseudoalterin served as the negative control. A, macroscopic observation of the hydrolysis of bovine elastin by pseudoalterin. B, light microscopic observation of hydrolysis of bovine elastin by pseudoalterin after 1 and 2 h. Samples were photographed with an inverted microscope (Olympus IX71, Japan) at room temperature. Magnification is ×960.