FIGURE 3.

Analysis of the cleavage sites of pseudoalterin on the synthetic peptides. Each of these peptides (5 μg) was mixed with 5 μg of pseudoalterin in 50 μl of 50 mm Tris-HCl (pH 9.0). After incubation at 25 °C for 600 min for GVGVAP, IGGGAGG, GIGLGP, and PGAGARF and 110 min for AAAKAA and AAAAAKAAAK, the reaction was terminated by the addition of 1% trifluoroacetic acid. The molecular masses and sequences of peptides were analyzed by LC-MS and the ExPASy FindPept Tool, as shown in supplemental Fig. S9.