FIGURE 5.

Cleavage sites by pseudoalterin on bovine tropoelastin (SwissProt accession number P04985-1). Cleavage sites were determined by analysis of the sequences of peptides released by pseudoalterin from bovine elastin as shown in supplemental Table S3, and the cleavage sites of pseudoalterin on six peptides were synthesized according to the sequence of bovine tropoelastin as shown in Fig. 3. Hollow arrows indicate the cleavage sites that have not been identified for other M23 proteases. Solid arrows indicate the cleavage sites that have been reported for other M23 proteases. Gly-Leu bond was reported to be cleaved by Lysobacter β-lytic protease (23). Gly-Gly and Gly-Ala bonds were reported to be cleaved by both Achromobacter β-lytic protease (15) and staphylolysin (29).