. 2012 Sep 25;287(47):39710–39720. doi: 10.1074/jbc.M112.405076

TABLE 2.

Effects of metal ions and inhibitors on the elastolytic activity of pseudoalterin

PAPMSF is p-amidinophenylmethylsulfonyl fluoride.

Metal ion (2 mm)	Relative activity^a	Metal ion (2 mm)	Relative activity^a	Inhibitors	Residual activity^b
	%		%		%
Control	100	Ni²⁺	71.3	Control	100
Ca²⁺	287.3	Mn²⁺	64.5	PAPMSF	110.0
Sr²⁺	228.3	Cu²⁺	63.3	Leuhistin	92.9
Mg²⁺	104.3	Sn²⁺	63.2	EDTA	13.4
K⁺	95.9	Fe²⁺	49.1	1,10-Phenanthroline	0
Na⁺	94.4	Zn²⁺	2.8
Co²⁺	86.7

^a Elastolytic activity of pseudoalterin was measured at 25 °C for elastinorcein. The activity of pseudoalterin without any metal ion was used as a control (100%). Activity was expressed as the activity of pseudoalterin with a metal ion relative to the activity with the control. The data are the means of three experiments, and the standard deviations were ≤5%.

^b Pseudoalterin was incubated with each inhibitor at 25 °C for 20 min, and then the enzyme activity was measured at 25 °C for elastinorcein. The activity of pseudoalterin without any inhibitor was used as the control (100%). Activity was expressed as the activity of pseudoalterin treated with an inhibitor relative to the control activity. The data are the means of three experiments, and the standard deviations were ≤5%.