FIGURE 2.
Inhibition of PARP activity results in retention of UVR-induced lesions. HaCaT cells were pre-exposed to a PARP inhibitor, DPQ, 30 min prior to a single dose of ssUVR (3 kJ/m2) or exposed to UVR alone and collected at the indicated times postexposure. A, quantification of PAR Western blots by densitometry. The data are presented as the means ± S.E., n = 3. B, quantification of 6-4 PPs obtained from immunofluorescent images. UV only samples (open triangles) and DPQ+UV samples (closed circles). Fluorescence intensity was normalized to untreated (NT) sample. The data are presented as the means ± S.E., n = 4. C, quantification of CPDs obtained from immunofluorescent images. Fluorescence intensity of raw data is shown. The data are presented as the means ± S.E., n = 3. *, p < 0.05; **, p < 0.01; ***, p < 0.01.