FIGURE 3.

Endogenous RNP-associated RNA sequences with regions of sequence complementarity can activate AM14 B cells independently of TLR7. A–F, AM14 □ or AM14 TLR7-deficient ■ B cells were stimulated with 1D4 premixed with the indicated bio-RNAs. Proliferation is plotted as the percent of the response to CpG-B. Average ± S.E. is shown, A and C, n = 4, B and D–F, n = 3. G, percent of proliferation dependent of TLR7 from A–F, plotted against the number of base-paired guanines predicted by MFOLD. Stimulatory RNAs (●) were compared for the linear regression, R² = 0.6575, p < 0.0001, weakly stimulatory (<10% Proliferation on WT) RNAs (Δ) were not included in this analysis. H, qRT-PCR for TLR3 from resting and 1 h IFN-primed AM14 □ or AM14 TLR7 KO ■ B cells. Data represented as ΔΔCT fold change over media, relative to GAPDH. Average ± S.E. is shown, n = 3.