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. 2012 Oct 3;287(47):39380–39390. doi: 10.1074/jbc.M112.406652

FIGURE 2.

FIGURE 2.

Ndc80 complex and Mis12 complex are required for the kinetochore localization of SKAP. A, representative images of metaphase HeLa cells transfected with CENP-E, Hec1, or Mis13 siRNA for 36 h followed by fixation and then labeled with antibodies against SKAP (red), tubulin (green), ACA (purple), and DNA (blue). Scale bar, 10 μm. B, quantification of kinetochore signal intensity of SKAP. Bars, mean ± S.E. (error bars) of three independent experiments. In each experiment, 20 cells were measured (≥15 kinetochores/cell). C and D, representative images of metaphase HeLa cells transfected with SKAP siRNA for 36 h followed by fixation and then labeled with antibodies against Hec1 or Mis13 (green), ACA (red), and DNA (blue). Scale bar, 10 μm.