FIGURE 5.

SKAP is required for accurate sister kinetochore oscillation. A, depletion of SKAP results in chromosome alignment errors. Immunofluorescence staining of control and SKAP siRNA-treated cells was conducted for ACA (red), tubulin (green), and DNA (blue). Scale bar, 10 μm. B, statistical analyses of phenotypes of SKAP-suppressed cells using two different siRNAs. C, HeLa cells expressing GFP-CENP-B were imaged 36 h after transfection of the indicated siRNAs (scramble, SKAP, and CENP-H); time is given in seconds. Paired sister kinetochores are color-coded to illustrate their dynamics. D, motion paths of color-coded sister kinetochores to the metaphase plate under various experimental conditions (transfection of scramble, CENP-H, and SKAP siRNAs individually) were traced during the given time. A kinetochore oscillatory kymograph is presented over time (seconds).