TABLE 1.
Distances (Å) between opposite S4-S5 linkers
Cross-pore distances (Å) for seven residues along the S4-S5 linker of a KvAP dimer were determined by LRET in the open and the closed state. Data are the average of at least n = 5 for each distance with S.D. as the error. Distances for the corresponding positions of Kv1.2/2.1 chimera were measured from the crystal structure (2R9R) in the open state and served as a comparison reference (in italics).
| Position | Closed | Open | Kv 1.2/2.1 | Position |
|---|---|---|---|---|
| F137C | 33.4 ± 2.7 | 42.2 ± 0.3 | 42.9 | G309C |
| L138C | 42.8 ± 0.9 | 46.7 ± 0.6 | 49.8 | L310C |
| S139C | 40.2 ± 0.4 | 44.3 ± 1.4 | 52.4 | Q311C |
| A140C | 30.1 ± 0.5 | 35.4 ± 0.1 | 38.0 | I312C |
| A142C | 37.9 ± 1.5 | 47.8 ± 0.3 | 48.3 | G314C |
| D143C | 33.2 ± 1.1 | 42.4 ± 0.1 | 39.8 | L315C |
| D146C | 35.9 ± 0.3 | 41.9 ± 0.6 | 44.4 | K318C |