FIGURE 8.

Differential scanning fluorimetry analysis of heparin derivatives on FGF-7 and FGF-9 reveals distinct dependence on substitution pattern. Differential scanning fluorimetry was performed with a range of heparin-based poly- and oligosaccharides with 5 μm protein and 175 μg/ml sugar. A, relative thermal stabilization effect of controls (PBS and heparin), chemically modified heparins (D2–D9), and other GAGs (HS, HA, CS, and DS). B, cation-modified heparin forms (20). C, heparin-derived oligosaccharides, ranging from DP2 to 12. Results are the mean of triplicates after normalization ± S.E., and an apparent absence of error bar is due to a small S.E.