TABLE 3.
Summary of peptides identified by Protect and Label structure proteomics
Labeled peptides were identified by tandem mass spectrometry and analyzed by Protein Prospector package Version 5.9.2. Here, a summary of the peptides involved in the heparin-binding sites and the labeled position is provided. A full list of identified peptides is provided in the (supplemental Table S1).
| FGF | Peptide | Sequences | HBSa | Residuesb |
|---|---|---|---|---|
| FGF-1 | 1 | VGLK(biotin)K(biotin)NGSC(carboxymethyl)K(biotin)RGPRTHY GQ(carboxymethyl)K(biotin)AILFLPL | 1 | 124–150 |
| 2 | ISK(biotin/acetyl)K(biotin/acetyl)HAEK(biotin/acetyl)NWF | 2/1 | 113–123 | |
| 3 | K(biotin/acetyl)K(biotin/acetyl)PK(biotin/acetyl)LLY | 3 | 24–30 | |
| 4 | IK(biotin)STETGQYL | 3 | 71–80 | |
| FGF-7 | 1 | VALNQK(biotin)GIPVRGK(biotin/acetyl)K(biotin/acetyl)TK (biotin/acetyl)K(biotin)EQK(acetyl)TAHF | 1 | 165–188 |
| 2 | LAM(oxidation)NK(biotin)EGK(acetyl)LY | 1 | 119–128 | |
| 3 | ASAK(biotin)WTHNGGEMF | 1 | 152–164 | |
| 4 | YLRIDK(biotin/acetyl)RGK(biotin/acetyl)VK(biotin/acetyl)GTQE MK(acetyl)NNY | 4 | 76–95 | |
| FGF-9 | 1 | VALNK(biotin)DGTPREGTRTK(biotin)RHQK(biotin)F | 1 | 164–184 |
| 2 | HLEIFPNGTIQGTRK(biotin)DHSRF | 1 | 73–92 | |
| 3 | THFLPRPVDPDK(biotin)VPELY | 1 | 185–201 | |
| 4 | K(biotin)HVDTGRRY | 1 | 154–162 | |
| FGF-18 | 1 | TK(biotin)K(biotin)GRPRK(biotin)GPK(biotin)TRENQQDVHFM (oxidation) | 1 | 154–175 |
| 2 | C(carbamidomethyl)MNRK(biotin/acetyl)GK(biotin)LVGK | 1 | 109–129 | |
| (Biotin/acetyl)PDGTSK(biotin/acetyl)EC(carbamidomethyl)VF | ||||
| 3 | RIHVENQTRARDDVSRK(biotin)QL | 2 | 34–52 | |
| 4 | GRRISARGEDGDK(biotin)Y | 2 | 70–83 | |
| 5 | GSQVRIK(biotin/acetyl)GK(biotin/acetyl)ETEFYL | 2 | 94–108 | |
| 6 | M(oxidation)K(biotin/acetyl)RYPK(biotin/acetyl)GQPELQK (biotin/acetyl)PF | 2 | 175–189 |