FIGURE 2.

Influences of GCN5-deficiency on sensitivity to various DNA-damaging agents. A, cell viability. DT40 (circles) and GCN5^−/− clone 1 (squares) were cultured with various DNA-damaging agents; 20 μm APC, 400 μg/ml BLEO, 30 μm CDDP, 100 nm CPT, 20 μm ETO, 1 μg/ml MMC, or 130 μg/ml MMS at 37 °C up to indicated times, respectively. Viable cells were counted by the trypan blue dye exclusion method. Data represent the average of three separate experiments. B, DNA fragmentation. DT40 (+) and GCN5^−/− clone 1 (−) were cultured with various DNA-damaging agents; 20 μm APC (6 h), 400 μg/ml BLEO (6 h), 30 μm CDDP (24 h), 100 nm CPT (6 h), 20 μm ETO (6 h), 1 μg/ml MMC (24 h), or 130 μg/ml MMS (8 h) at 37 °C. DNAs were isolated from the cells and analyzed by 1.5% agarose gel electrophoresis. The sizes of λ-DNA digested with HindIII are indicated in kilobase pairs.