FIGURE 3.

Influences of GCN5-deficiency on expression of NER- and TLS-related genes. A, XP-related genes. B, other NER- and TLS-related genes. Total RNAs were extracted from DT40 and three independent GCN5^−/− clones, and mRNA levels of NER- and TLS-related genes found in chicken were determined by semiquantitative RT-PCR using appropriate primers (see supplemental Table S1). Chicken GAPDH gene was used as an internal control for calibration. Data represent averages of three independent GCN5^−/− clones (solid bars), and indicated as percentages of control values (100%) obtained from DT40 cells (gray bars). Error bars indicate S.D. Statistical differences were calculated using t test. *, p < 0.05; **, p < 0.01.