FIGURE 4.

NRG4 does not stimulate colonocyte proliferation or migration. A, YAMC-B4 cells were given NRG4 or EGF (10 ng/ml, positive control for cell stimulation) for 24 h and then labeled with EdU to determine proliferative index. The graph depicts results from three independent experiments. *, p < 0.01 versus all other columns. B, fixed colon sections from PBS- or NRG4-injected mice were immunostained for the proliferative marker Ki67, and the number of labeled cells/crypt was counted. Data points are average cells/crypt in individual mice. n.s., not significant. C, YAMC-B4 cells were subjected to an 8-h migration/restitution assay in the presence of NRG4 or EGF. *, p < 0.01 versus all other columns. Error bars, S.E.